acillus safensis fo 36b 84 2  (ATCC)

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: List of B. safensis FO-36b genes not shared by B. pumilus SAFR-032, B. pumilus ATCC7061 T and B. safensis JPL_MERTA8-2

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques: CRISPR

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: B. safensis F0-36b unique genes

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: The Bacillus bacteriophage SPP1 (NC_004166) homologous region in the B. safensis FO-36b genome, as compared with the equivalent genomic regions of B. pumilus ATCC7061 T , B. pumilus SAFR-032 and B .subtilis subsp. subtilis str. 168. The locus tag numbers are given inside the boxes/rectangles. Red diamonds denote absence of a single gene/homolog. Red rectangle denotes absence of a series/cluster of ORFs/genes. Green box encloses the phage insertion region. Green diamond denotes absence of a single gene/homolog within the phage. “hyd” = hydrolase, “chp” = conserved hypothetical protein, “pept” = peptidase, “hp” = hypothetical protein, “TR” = transcriptional regulator, “Ps” = pseudogene, “lp” = lipoprotein, “gsp” = group specific protein, “oxi” = oxidase

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: The Bacillus bacteriophage SPP1 (NC_004166) homologous region in the B. safensis FO-36b genome, as compared with the equivalent genomic region of B. safensis JPL_MERTA8–2. Red diamonds denote absence of a single gene/homolog. Red rectangle denotes absence of a series/cluster of ORFs/genes. Green box encloses the phage insertion region

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: Overall scheme of the Brevibacillus phage Jimmer1 (NC_029104) phage insertion in the B. safensis FO-36b genome. The three blocks A, B and C and the genes they encompass are shown. The first part of Block C is a duplication of Block A

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques: Blocking Assay

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: The Brevibacillus phage Jimmer1 (NC_029104) phage insertion in the B. safensis FO-36b genome as compared with the equivalent region in the genome of B. pumilus ATCC7061 T . Black box encloses the phage insertion region(s). Green (dashed line) box corresponds to block A. Green (dotted line) box corresponds to block B. Blue (dashed line) box corresponds to block C. Red (dashed line) box encloses ‘terminase’ genes. A diamond denotes absence of a single gene/homolog within the phage, while rectangle denotes absence of a cluster of genes/homologs. “hp” = hypothetical protein, “chp” = conserved hypothetical protein, “pp” = phage portal protein, “sp” = structural protein, “sgp” = spore germination protein, “int” = integrase

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques: Blocking Assay

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: The Brevibacillus phage Jimmer1 (NC_029104) phage insertion in the B. safensis FO-36b genome as compared with the equivalent region in the genome of B. safensis JPL_MERTA8–2. “hp” = hypothetical protein, “chp” = conserved hypothetical protein, “pp” = phage portal protein, “sp” = structural protein, “sgp” = spore germination protein, “int” = integrase

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: The Brevibacillus phage Jimmer1 (NC_029104) phage insertion in the B. safensis FO-36b genome as compared with the equivalent region in the genome of B. pumilus SAFR-032. “hp” = hypothetical protein, “chp” = conserved hypothetical protein, “pp” = phage portal protein, “sp” = structural protein, “sgp” = spore germination protein, “int” = integrase.

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: The Brevibacillus phage Jimmer1 (NC_029104) phage insertion in the B. safensis FO-36b genome as compared with the equivalent region in the genome of B. subtilis . “hp” = hypothetical protein, “chp” = conserved hypothetical protein, “pp” = phage portal protein, “sp” = structural protein,“sgp” = spore germination protein, “int” = integrase

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: Whole genome Phylogenetic Analysis (WGPA) using the latest version of the Genome-BLAST Distance Phylogeny (GBDP). B. safensis FO-36b, B. safensis JPL_MERTA8–2B, B. pumilus SAFR-032, and B. pumilus ATCC7061 T are highlighted in red dash-lined rectangles

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: Molecular Phylogenetic analysis by the Maximum Likelihood method. B. safensis FO-36b, B. safensis JPL_MERTA8–2B, B. pumilus SAFR-032, and B. pumilus ATCC7061 T are highlighted in red dash-lined rectangles

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques:

Journal: BMC Microbiology

Article Title: Bacillus safensis FO-36b and Bacillus pumilus SAFR-032: a whole genome comparison of two spacecraft assembly facility isolates

doi: 10.1186/s12866-018-1191-y

Figure Lengend Snippet: B. safensis FO-36b genes (hypothetical proteins) with fewer than 5 homologs

Article Snippet: 5 μg of purified genomic DNA of FO-36b was digested with NEBNext dsDNA Fragmentase (New England Biolabs, Ipswich, MA) yielding dsDNA fragments in a size range of 50 bp up to 1000 bp.

Techniques: